Abstract: Defending Our National Treasure: A Department of Defense Chesapeake Bay Restoration Partnership 1998–2004 provides an overview of major issues impacting the Chesapeake Bay, history of the Department of Defense’s involvement in Bay restoration efforts, current Department of Defense Chesapeake Bay restoration initiatives, specific case studies, and viewpoints of various key individuals dedicated to restoration. These topics are presented in a richly illustrated style including maps, photographs, conceptual diagrams, and figures to uniquely communicate information and make it accessible to a broad audience. Each section provides the essence of each topic rather than the complete and comprehensive treatment. For example, there are numerous documents describing Chesapeake Bay and Department of Defense initiatives (www.denix.osd.mil). Defending Our National Treasure provides the context and background for the issues impacting the Chesapeake Bay and describes the restoration activities conducted on the Department of Defense installations within the Bay watershed.

Abstract: Clostridium perfringens in sediment samples collected at the Deep Water Municipal Sewage Sludge Disposal Site (also called the 106-Mile Site), off the coast of New Jersey, was enumerated. The counts of C. perfringens found in sediment samples collected within and to the southwest of the 106-Mile Site were significantly elevated (P < 0.01) compared with counts of samples from reference stations of similar depth (2,400 to 2,700 m), topography, and distance from the continental shelf, indicating that the benthic environment was contaminated by sewage dumping at this site. Low counts of C. perfringens in sediment samples collected at stations between the base of the continental shelf and the 106-Mile Site indicated that coastal runoff was not a significant source of contamination. Elevated counts were observed for samples up to 92 km to the southwest, whereas low counts were obtained for samples from stations to the east of the 106-Mile Site. This distribution is consistent with previous model predictions of sludge deposition. In areas heavily impacted by sludge dumping, C. perfringens counts were generally highest in the top 1 cm of sediment and exceeded 9,000 CFU g (dry weight) of sediment-1. The patterns of C. perfringens dispersal observed in this study have proved use heavily impacted areas and control stations for further ecological evaluation by a multidisciplinary research team.

Abstract: Chesapeake Bay was investigated as a source of actinomycetes to screen for production of novel bioactive compounds. The presence of relatively large populations of actinoplanetes (chemotype II/D actinomycetes) in Chesapeake Bay sediment samples indicates that it is an eminently suitable ecosystem from which to isolate actinomycetes for screening programs. Actinomycetes were isolated from sediment samples collected in Chesapeake Bay with an isolation medium containing nalidixic acid, which proved to be more effective than heat pretreatment of samples. Actinomycete counts ranged from a high of 1.4 x 10(5) to a low of 1.8 x 10(2) CFU/ml of sediment. Actinomycetes constituted 0.15 to 8.63% of the culturable microbial community. The majority of isolates from the eight stations studied were actinoplanetes (i.e., chemotype II/D), and 249 of these isolates were obtained in a total of 298 actinomycete isolates. Antimicrobial activity profiles indicated that diverse populations of actinoplanetes were present at each station. DNA hybridization studies showed considerable diversity among isolates between stations, but indicated that actinoplanete strains making up populations at nearby stations were more similar to each other than to populations sampled at distant stations. The diversity of actinoplanetes and the ease with which these organisms were isolated from Chesapeake Bay sediments make this a useful source of these actinomycetes.